Do not use abrasive or chemical detergents to clean the phocheck tiger instrument as this may reduce the antistatic properties of the materials. National instruments gpib232 user manual pdf download. The uninstall ettan ipgphor ii control software program will start. Ettan ipgphor 3 ief system is a fully integrated isoelectric focusing system. Operation manual for the ta instruments dsc q100 and q20. Manuals for products and instruments castle group ltd. To ensure complete sample uptake, do not apply excess rehydration solution. This page shows how to perform isoelectric focusing with ettan ipgphor 3 isoelectric.
Selective labelling of cellsurface proteins using cydye dige. There are so many interesting aspects to what we do that we though our customers might like to hear about some of them. Ettan ipgphor 3 control software user manual 11003459. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3. Read and understand this user manual completely before operating the phocheck tiger instrument. Ettan ipgphor isoelectric focusing system ipgphor function and description um 80641535 p2 control panel negative cathode electrode area positive anode electrode area cover pressure block sample application wells base 1. Manuals for products and instruments manufactured and supplied by castle group. Ettan ipgphor ii manualzz owner guides and user manuals. Ettan dige system user manual 18117317 edition ab v. Ettan ipgphor 3 control software allows easy programming on a pc fig 2. The ettan dalt ii system is designed to handle multiple large seconddimension gels in a simple, ef. The ettan ipgphor 3 control panel allows up to 10 user. National instruments shall not be liable for any delay in performance due to causes beyond its reasonable.
Ettan ipgphor manifold polymeric tray thomas scientific. User configurable values include the baudrate and slave address. Ge healthcare ettan ipgphor 3 isoelectric focusing system. Printing and saving of the data to a text or spreadsheet file. This instrument automatically shuts off after 3 0 minutes of. Check out our newsletter to read about what the team have been getting up to. This manual is written with operation of the ij7 demo module in mind to allow the user to reproduce any of the graphs shown, but the underlying principles are applicable to any temperature control system. I would much appreciate to receive a pdf of your paper. At682683 users manual 2 safety summary warning dangerous. Ettan ipgphor 3 isoelectric focusing system function and description 1 ettan ipgphor 3 instrument user manual 11003458 edition ab 9 1.
Instrument components five strip holder lengths are available 7, 11, 18, and 24 cmone for each ipg. Any action against national instruments must be brought within one year after the cause of action accrues. Fully integrated equipment optimized to deliver high throughput, speed, reproducibility and high proteinloading capacity. The word cautionis used to indicate conditions or actions that may damage this instrument. Please contact applent instruments incorporation sales representative for repair of the instrument. Programmable functions include rehydration time, platform temperature, current limit, voltage limit for each step, voltage step or gradient, and step duration. User friendly ettan ipgphor 3 control software is a user friendly interface to the ettan ipgphor. Data file 28404198 aa ettan ipgphor 3 ettan ipgphor 3 isoelectric focusing system the ettan ipgphor 3 is a fully integrated isoelectric focusing ief system optimized to deliver high throughput, ease of use, speed, reproducibility, and high proteinloading capacity. Guide for finding information on gel casting for daltsix and dalttwelve. Close the lid of the ipgphor 3 and connect the instrument to the control software if. If you do not have a pdf reader, you can download one here. The system components include the ettan daltsixelectrophoresis unit, gel caster, gradient maker, an eps 601 power. Recently, we reported the full sequences and properties of two acidic g.
Ettan daltsixelectrophoresis unit are maintained by the combination of the builtin ceramic heat exchanger and buffer circulation pump with an external multitemp iii thermostatic circulator. Ipgphor 3 instrument user manual and ettan ipgphor 3 safety handbook prior to operation of the. When you notice any of the unusual conditions listed below, immediately terminate operation and disconnect the power cable. Seal the ipg strip in place with agarose sealing solution. Call 18004biorad or your local biorad representative for technical information regarding additional chemical compatibility of the miniprotean 3 cell with various laboratory reagents. Precast dige gels and dige buffer kit were used for the seconddimension run in ettan dalttwelve electrophoresis unit according to the dige gel instruction. Instrument components control panel the mains power switch is located on the back panel on the left side. Perform the electrophoresis using the ettan dalttwelve system at 5 wgel for 30 min and then at 28 wgel until the bpb dye front just leaves the gel. Read the instruction manual fully before starting up the um3b unit. Enduser license agreements and thirdparty legal notices you can find enduser license agreem ents eulas and thirdparty legal notices in the following locations. Setting the slave address to 255 will trigger a reset event, and all settings will revert back to the original default, which is slave address 1 i.
The protocol of 2de with ipgs has been constantly refined, e. Ettan ipgphor 3 control software user manual 11003459 ettan ipgphor 3 instrument user manual 11003458 ettan ipgphor cup loading manifold user manual 11003460 2d electrophoresis principles and methods 80642960 ettan dalttwelve system user manual 80647653. The strips were run in a manifold on an ettan ipgphor 3 instrument. Instruction manuals are in the portable document format pdf. These features allow the user to explore the operation of the ij6 controller. The default installation of the ettan ipgphor ii control software will be on the local c drive within the folder titled amersham biosciences. For detailed instructions on installation and usage of this software, please refer to the ettan ipgphor ii control software user manual 80650047. National instruments 6023e user manual pdf download. User friendly ettan ipgphor 3 control software is a userfriendly interface to the ettan ipgphor. I can not load it from jove web site selective labelling of cellsurface proteins using cydye dige fluor minimal dyes. Ni product manuals find technical documentation for ni products including product manuals, getting started guides, and specifications.
All pdf files can be viewed with the adobe acrobat reader, which is a free download from adobe. Manuals support discussion forums downloads examples knowledgebase product support pages tutorials entire site. Ettan ipgphor 3 control software user manual 11003459 edition ab 7 1introduction 1. Countess ii and countess ii fl automated cell counters. The gels were stored overnight at 4c before scanning in typhoon fla 9000 biomolecular imager. The ettan ipgphor 3 control panel allows up to 10 userdefined ief protocols, each with nine steps.
Negative cathode electrode area positive anode electrode area five strip holder lengths are available 7,11, 18, and 24 cmone for each ipg strip length. These are pdf files, therefore you will need adobe reader for viewing. In this manual the word warningis used to indicate conditions or actions that may pose physical hazards to the user. The ettan ipgphor 3 control panel allows up to 10 user defined ief protocols, each with nine steps.
Safety read the following safety information carefully before attempting to operate the meter. User manual hard carrying case safety notes before using this meter, read all safety information carefully. Because ettan dige system comprises many different products, references to the appropriate manuals for further details are made when necessary, see table 11. Control software and uploaded to the instrument instantly. Inserting gels into the ettan dalt electrophoresis buffer tank. The user can create, save, and edit protocols and also pause during a run to edit a. Ief using ettan ipgphor 3 isoelectric focusing system and. Additional requirements for instrument control software description requirements user login capability while multiple users may still use the fast user switching function when running windows xp or vista, only one user at a time may use the ta instruments q series tm, thermal, or rheology advantage instrument control software. Ettan ipgphor 3 is a highvoltage instrument that can cause fatal electrical shock if the safety features are disabled. In order to view these files, you will need the adobe acrobat reader, which is available for free from adobe web site. View and download national instruments 6023e user manual online.
Miniprotean 3 components are not compatible with acetone, ethanol, or butanol. Important user information please read this entire manual to fully understand the safe and effective use of this product. Imaging twodimensional protein gels stained with sypro ruby. For more details, refer to ettan dalttwelvesystem user manual and the 2d electrophoresis technical manual 1. For detailed instructions on installation and usage of this software, refer to the ettan ipgphor 3 control software user manual. Transcriptome and proteome of the highly neurotoxic venom of. The following sections will guide you through each stage of the isoelectric focusing. Ettan dige system users manual university of alberta. Ettan daltsixelectrophoresis system function and description in 2d electrophoresis, proteins are separated according to isoelectric point by isoelectric focusing, most reliably on immobiline drystrip immobilized ph gradient ipg gel strips using the ipgphor or multiphor ii ief systems. Multifunction io devices for pci, pxi, compactpci, and pcmcia bus computers. Ettan ipgphor3 ief system 7cm strip protocols youtube. Hagnermcwhirter a, winkvist m, bourin s, marouga r.
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